模式动物嘉宾摘要抢先看！

随着基因编辑技术的发展，疾病动物模型的建立方法更加精准和快捷。为进一步落实国家精准医学计划，疾病动物模型作为实验医学研究的一项基础性工作将面临更严格的要求。为此，生物谷举办2018（第二届）模式动物与重大疾病动物模型研究与应用研讨会。希望通过本次会议推动我国在疾病动物模型方面的基础与转化医学研究，为医学科学研究基础平台建设打下基础。

截止今天，嘉宾已确认了大半，今天小编为大家准备了3位嘉宾老师的演讲摘要，让各位看官一睹为快！如果觉得还不错，那就赶快报名吧~

陈策实研究员

中国科学院昆明动物研究所

演讲题目：树鼩乳腺癌模型研究

演讲摘要：

树鼩是一种接近灵长类的哺乳动物，频繁自发乳腺癌。在完成树鼩基因组测序的基础上，我们对树鼩自发乳腺癌进行了病理和遗传分析，发现树鼩乳腺癌多为导管内乳头状瘤，频繁发生pTEN/PI3KCA基因突变。我们采用致癌剂DMBA可以诱导树鼩发生乳腺癌，人工合成孕激素可以提高效率。采用慢病毒乳头注射方法表达病毒癌基因PyMT可以在1个月左右几乎100%诱导树鼩产生乳腺癌，这种模型对临床化疗药物顺铂和表阿霉素有良好反应。我们还对树鼩KLF转录因子家族、树鼩端粒以及端粒酶进行了研究。这些研究结果为发展树鼩乳腺癌模型，建立新的药物评价系统，以及乳腺癌基础研究奠定了基础。

许执恒研究员

中科院遗传发育所

演讲题目：Zika Virus Infection Incurred Microcephaly, Pathogenesis and Treatment

演讲摘要：

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences. email: zhxu@genetics.ac.cn.

The link between Zika virus (ZIKV) infection and microcephaly has raised urgent global alarm. We have provided the first direct evidence for the causal link between Zika virus (ZIKV) infection and microcephaly (Li et al., Cell Stem Cell 2016). We found that Asian ZIKV strain replicates efficiently in embryonic mouse brain by directly targeting different neuronal linages. ZIKV infection leads to cell cycle arrest, apoptosis and inhibition of NPC differentiation, resulting in cortical thinning and microcephaly. However, whether glial cells which constitute more than half of the brain cells are affected, is unclear. Here we show that ZIKV infects the glial progenitors during later stage of brain development and leads to severe microcephaly in neonatal mice. ZIKV infection significantly disturbs the proliferation and differentiation of glial precursors. Notably, global gene expression analysis of infected brains reveals the dysregulation of large numbers of genes associated with brain development including oligodendrocyte development, in addition to immune response, neurogenesis and apoptosis. Our results indicate that the disrupted development of glial cells accounts for ZIKV infection incurred microcephaly. Our new model would provide more insights into the pathogenesis of ZIKV infection and valuable information for the treatment of related pathological effects. More importantly, I will discuss why the concurrent ZIKV Asian strains, but not the Africa strain, can cause microcephaly as well as the treatment of ZIKV infection.

张文清教授

华南理工大学医学院

演讲题目：斑马鱼白血病模型构建与药物筛选

演讲摘要：

白血病是一类造血干细胞异常的克隆性恶性疾病，15岁以下患有癌症的儿童有1/3是白血病。更重要的是，90%以上白血病的致病原因和发病机理都是不明确的。白血病基因治疗的关键是确定不同类型白血病的不同致病基因。造血关键性步骤是通过各种转录因子如PU.1、cMyb、CEBPα和RUNX1等之间的相互作用实现的。斑马鱼具有体积小、生殖能力强、生殖周期短、体外受精、胚胎透明且生长迅速等独特的优势，特别适用于正向遗传学突变体的筛选；同时，斑马鱼的血液系统早期发育与人类极为相似，造血缺陷突变体可存活数天。因此，我们选择斑马鱼独特作为脊椎动物髓系造血研究模型，通过ENU化学诱变大规模（3000个基因组）正向遗传学筛选和TILLING、TALEN或Cas9反向遗传学特异性敲除/敲入斑马鱼白血病致病基因筛选获得30余种造血缺陷突变体并建立5个不同类型的白血病斑马鱼模型，以期阐明造血新的分子机制、寻找白血病临床早期诊断、预后和治疗效果评判的分子标记物以及开展基于斑马鱼白血病模型的活体高通量药物筛选和基因治疗研究。